{"id":2363,"date":"2026-10-14T23:00:15","date_gmt":"2026-10-14T15:00:15","guid":{"rendered":"http:\/\/www.puriactives.com\/en\/?p=2363"},"modified":"2026-10-14T23:00:15","modified_gmt":"2026-10-14T15:00:15","slug":"nanoactive-oleu","status":"publish","type":"post","link":"http:\/\/www.puriactives.com\/en\/?p=2363","title":{"rendered":"NanoActive Oleu™"},"content":{"rendered":"

NanoActive Oleu™<\/b><\/h2>\n

\"\"<\/p>\n

PuriPharm Co., Ltd. \u00b7 Technical Report<\/b><\/p>\n

Liposomal Oleuropein<\/b><\/p>\n

Mediterranean polyphenol defense<\/p>\n

Enhanced by delivery science<\/p>\n

INCI: OLEA EUROPAEA (OLIVE) LEAF EXTRACT<\/p>\n

Powered by PURISOME\u00ae FlexVes™ Liposome Technology<\/p>\n

01 \u00b7 INGREDIENT OVERVIEW<\/p>\n

NanoActive Oleu™ at a glance<\/b><\/h2>\n

PRODUCT NAME\uff1aNanoActive Oleu™ Liposomal Oleuropein<\/b><\/p>\n

INCI NAME\uff1aOlea Europaea (Olive) Leaf Extract<\/b><\/p>\n

BOTANICAL SOURCE\uff1aLeaves of Olea europaea L.<\/b><\/p>\n

KEY PHYTOCHEMICAL\uff1aOleuropein, a secoiridoid polyphenol<\/b><\/p>\n

DELIVERY PLATFORM\uff1aPURISOME\u00ae FlexVes™ Liposome Technology<\/b><\/p>\n

PHYSICAL FORM\uff1aLiposomal dispersion for aqueous and emulsified systems<\/b><\/p>\n

APPLICATION AREAS\uff1aSkincare \u00b7 Oral care \u00b7 Hair and scalp care<\/b><\/p>\n

PRODUCT POSITIONING\uff1aAn oleuropein-rich olive leaf extract encapsulated in flexible liposomal vesicles. NanoActive Oleu™ brings Mediterranean polyphenol science to formulation-ready skin, oral and scalp care concepts, informed by human, in vivo and mechanistic research.<\/b><\/p>\n

\"\"<\/p>\n

Young olive leaves (Olea europaea L.), the botanical source of the actives.<\/p>\n

02 \u00b7 BOTANICAL ORIGIN<\/p>\n

Olive leaves: a concentrated source of polyphenols<\/b><\/h2>\n

Olive branch with young leaves and fruit. Leaves are the tree\u2019s richest source of phenolic compounds.<\/p>\n

60\u201390 mg oleuropein per g of dry leaves<\/b><\/p>\n

The olive tree concentrates its defensive chemistry in its leaves. Oleuropein, the predominant secoiridoid polyphenol, can reach 60\u201390 mg\/g of dry leaves, alongside hydroxytyrosol, verbascoside and flavonoid glycosides.<\/p>\n

This phytochemical system evolved under sunlight, microbial and oxidative stress, providing a botanical rationale relevant to daily challenges faced by skin, oral mucosa and scalp.<\/p>\n

Nediani C, et al. Antioxidants. 2019;8(12):578. doi:10.3390\/antiox8120578<\/p>\n

Omar SH. Sci Pharm. 2010;78(2):133\u2013154.<\/p>\n

03 \u00b7 KEY ACTIVE MOLECULE<\/p>\n

Oleuropein: secoiridoid chemistry for redox activity<\/b><\/h2>\n

\"\"<\/p>\n

Oleuropein (molecular weight 540.5): hydroxytyrosol + elenolic acid + glucose. Nediani C, et al. Antioxidants. 2019;8:578.<\/p>\n

CATECHOL CORE<\/b><\/p>\n

The catechol hydroxyls of the hydroxytyrosol moiety donate hydrogen atoms to neutralize free radicals.<\/p>\n

ACTIVE PRODUCTS OF HYDROLYSIS<\/b><\/p>\n

Ester hydrolysis releases hydroxytyrosol and elenolic acid, extending the molecule\u2019s functional profile.<\/p>\n

NATURAL HYDROPHILICITY<\/b><\/p>\n

A water-soluble glycoside with biological activity, but limited passage through lipid-rich barriers without delivery support.<\/p>\n

This chemistry provides the rationale for complementary delivery engineering.<\/p>\n

04 \u00b7 DELIVERY CHALLENGES<\/p>\n

Active polyphenols face practical delivery challenges<\/b><\/h2>\n

CHEMICAL INSTABILITY<\/b><\/p>\n

Oleuropein\u2019s ester bonds and catechol groups are susceptible to hydrolysis and oxidation, potentially reducing activity during shelf life.<\/p>\n

BARRIER MISMATCH<\/b><\/p>\n

Hydrophilic glycosides partition poorly into lipid-rich stratum corneum and mucosal surfaces, limiting local bioavailability.<\/p>\n

SHORT RESIDENCE TIME<\/b><\/p>\n

Unencapsulated polyphenols can be diluted, metabolized or washed away quickly, particularly in the oral environment.<\/p>\n

FORMULATION CONSTRAINTS<\/b><\/p>\n

Oxidation-sensitive polyphenols constrain pH, processing, packaging and preservative-system design.<\/p>\n

DELIVERY APPROACH<\/b><\/p>\n

Delivery science aims to turn a fragile molecule into a robust, formulation-ready ingredient.<\/p>\n

PURISOME\u00ae FlexVes™ Liposome Technology<\/b><\/p>\n

The proprietary liposomal platform behind the NanoActive™ ingredient range is introduced below.<\/p>\n

Stability reference: Perugini P, et al. Int J Cosmet Sci. 2008;30(2):113\u2013120.<\/p>\n

05 \u00b7 DELIVERY PLATFORM<\/p>\n

PURISOME\u00ae FlexVes™ Liposome Technology<\/b><\/h2>\n

Flexible phospholipid vesicles: PuriPharm\u2019s delivery architecture.<\/p>\n

PuriPharm\u2019s proprietary platform encapsulates oxidation-sensitive polyphenols in flexible liposomal vesicles, with four proposed formulation benefits.<\/p>\n

01 \u00b7 PROTECTION<\/b><\/p>\n

Protect actives vulnerable to oxidation and degradation to support shelf life.<\/p>\n

02 \u00b7 PENETRATION<\/b><\/p>\n

Promote stratum corneum penetration and improve bioavailability.<\/p>\n

03 \u00b7 RELEASE<\/b><\/p>\n

Provide sustained, controlled release to reduce irritation risk.<\/p>\n

04 \u00b7 COMPATIBILITY<\/b><\/p>\n

Improve formulation compatibility and skin feel.<\/b><\/p>\n

PuriPharm Co., Ltd. has more than ten years of liposome R&D experience.<\/p>\n

06 \u00b7 INTEGRATED VALUE<\/p>\n

One ingredient, a complete value framework<\/b><\/h2>\n

01<\/b><\/p>\n

OLIVE LEAVES<\/b><\/p>\n

BOTANICAL SOURCE<\/b><\/p>\n

Olea europaea L. leaves, the tree\u2019s most polyphenol-rich plant part.<\/p>\n

02<\/b><\/p>\n

PHYTOCHEMISTRY<\/b><\/p>\n

ACTIVE FOUNDATION<\/b><\/p>\n

An oleuropein-rich polyphenol system with published evidence across multiple pathways.<\/p>\n

03<\/b><\/p>\n

PURISOME\u00ae<\/b><\/p>\n

DELIVERY ENGINEERING<\/b><\/p>\n

FlexVes™ liposomal protection, penetration and controlled release.<\/p>\n

04<\/b><\/p>\n

FORMULATION FIT<\/b><\/p>\n

FLEXIBLE USE<\/b><\/p>\n

Dispersion for incorporation into emulsions, gels, serums and oral-care bases.<\/p>\n

05<\/b><\/p>\n

SKIN \u00b7 ORAL \u00b7 SCALP<\/b><\/p>\n

APPLICATION AREAS<\/b><\/p>\n

Three care areas, each with its own relevant evidence base.<\/b><\/p>\n

Botanical chemistry informs delivery engineering, delivery supports formulation design, and formulations translate the concept into three care areas.<\/p>\n

07 \u00b7 MECHANISM OVERVIEW<\/p>\n

Five pillars of cellular defense<\/b><\/h2>\n

WHY FIVE PILLARS?<\/b><\/p>\n

UV exposure, pollution, microorganisms and mechanical stress can occur together and converge on shared cellular mechanisms.<\/p>\n

Oleuropein research addresses this network from radical scavenging to cellular quality control.<\/p>\n

Mechanisms and supporting evidence are reviewed below.<\/b><\/p>\n

REDOX DEFENSE<\/b><\/p>\n

Direct radical scavenging and support for endogenous antioxidant systems.<\/p>\n

INFLAMMATORY MODULATION<\/b><\/p>\n

Modulation of NF-\u03baB-related signaling and key inflammatory mediators.<\/p>\n

CELLULAR QUALITY CONTROL<\/b><\/p>\n

Autophagy and proteostasis pathways involving AMPK, mTORC1, SIRT1 and TFEB.<\/p>\n

TISSUE REPAIR<\/b><\/p>\n

Support for re-epithelialization, collagen deposition and VEGF signaling.<\/p>\n

MICROBIAL BALANCE<\/b><\/p>\n

Published inhibitory activity against biofilm-forming, cariogenic oral bacteria.<\/p>\n

Mechanistic framework: Nediani C, et al. Antioxidants. 2019;8(12):578.<\/p>\n

07 \u00b7 MECHANISM: REDOX DEFENSE<\/p>\n

Quantifying radical-scavenging activity<\/b><\/h2>\n

IN VITRO EVIDENCE<\/b><\/p>\n

Oleuropein scavenges DPPH radicals in a concentration-dependent manner, with a curve approaching that of the reference antioxidant ascorbic acid. Complementary ABTS assays also support anti-radical activity.<\/p>\n

CHEMICAL RATIONALE<\/p>\n

\u00b7 Catechol hydroxyl groups donate hydrogen atoms to neutralize radicals.<\/p>\n

\u00b7 The resulting ortho-quinone structure is resonance-stabilized.<\/p>\n

\u00b7 Hydrolysis releases hydroxytyrosol, contributing further activity.<\/p>\n

In vitro radical-scavenging activity provides a rationale for using PURISOME\u00ae delivery to improve contact with sites of UV- and pollution-related oxidative stress.<\/p>\n

\"\"<\/p>\n

DPPH radical-scavenging activity: oleuropein (\u25cf) versus ascorbic acid (\u25cb, reference). Perugini P, et al. Int J Cosmet Sci. 2008;30(2):113\u2013120.<\/p>\n

Dose-dependent radical scavenging is the first mechanistic step, upstream of inflammation, barrier damage and visible aging.<\/p>\n

07 \u00b7 MECHANISM: INFLAMMATORY MODULATION<\/p>\n

Interrupting the oxidative stress\u2013inflammation cycle<\/b><\/h2>\n

\"\"<\/p>\n

Oxidative stress\u2013autophagy\u2013inflammation network. The indicated molecular markers are discussed in the oleuropein literature. Adapted from Nediani C, et al. Antioxidants. 2019;8(12):578.<\/p>\n

NF-\u03baB SIGNALING<\/b><\/p>\n

Modulated upstream of cytokine-cascade amplification.<\/b><\/p>\n

COX-2 AND iNOS<\/b><\/p>\n

These inflammatory enzymes are downregulated in experimental models.<\/p>\n

IL-6 \u00b7 TNF-\u03b1 \u00b7 IL-1\u03b2<\/b><\/p>\n

Key inflammatory mediators are reduced in cellular and tissue studies.<\/p>\n

ICAM-1 \u00b7 VCAM-1<\/b><\/p>\n

Reduced adhesion-molecule expression is associated with less immune-cell recruitment and inflammation.<\/p>\n

Oleuropein acts at the oxidative entry point of the cycle, providing an upstream intervention rationale.<\/p>\n

07 \u00b7 MECHANISM: CELLULAR QUALITY CONTROL<\/p>\n

Autophagy as a quality-control mechanism<\/b><\/h2>\n

Autophagy is the cellular recycling system that identifies and clears damaged proteins and organelles. Oleuropein has been reported as an autophagy inducer in several experimental models.<\/p>\n

01<\/b><\/p>\n

STRESS SENSING<\/b><\/p>\n

AMPK activation increases, while the mTORC1 brake on autophagy is reduced.<\/p>\n

AMPK \u2191 \u00b7 mTORC1 \u2193<\/b><\/p>\n

02<\/b><\/p>\n

AUTOPHAGY INITIATION<\/b><\/p>\n

Beclin-1 participates; LC3-I converts to LC3-II as autophagosomes form.<\/p>\n

Beclin-1 \u00b7 LC3-II \u2191<\/b><\/p>\n

03<\/b><\/p>\n

SUBSTRATE CLEARANCE<\/b><\/p>\n

p62 directs damaged proteins and organelles toward lysosomal degradation.<\/p>\n

p62 turnover \u2191<\/b><\/p>\n

04<\/b><\/p>\n

CELLULAR RENEWAL<\/b><\/p>\n

SIRT1 and TFEB support lysosomal biogenesis and cellular renewal.<\/p>\n

SIRT1 \u00b7 TFEB \u2191<\/b><\/p>\n

This cellular clearance mechanism provides a distinctive basis for botanical active development.<\/p>\n

Autophagy markers: Nediani C, et al. Antioxidants. 2019;8(12):578. doi:10.3390\/antiox8120578<\/p>\n

07 \u00b7 MECHANISM: PROTEOSTASIS<\/p>\n

Proteostasis support for lasting skin quality<\/b><\/h2>\n

\"\"<\/p>\n

THE PROTEOSTASIS PERSPECTIVE<\/b><\/p>\n

The proteostasis network, including synthesis, folding, repair and degradation, maintains functional proteins. Under chronic environmental stress, damaged material can accumulate faster than cells can clear it.<\/p>\n

Oleuropein is positioned on both sides of this balance: antioxidant defense slows damage formation, while autophagy induction supports clearance.<\/p>\n

Formulation implication: ingredients that support cellular quality control provide a rationale for long-term skin resilience as well as repair.<\/p>\n

Proteostasis and autophagy framework: Nediani C, et al. Antioxidants. 2019;8(12):578.<\/p>\n

08 \u00b7 SKINCARE: HUMAN EVIDENCE<\/p>\n

Human evidence under UVB stress<\/b><\/h2>\n

STUDY DESIGN<\/b><\/p>\n

MODEL<\/b><\/p>\n

UVB-induced erythema in human volunteers, a controlled photobiology challenge model.<\/p>\n

TEST FORMULATIONS<\/b><\/p>\n

Topical oleuropein emulsions and emulgels applied after UVB exposure.<\/p>\n

REFERENCE<\/b><\/p>\n

Vitamin E, a topical antioxidant benchmark.<\/b><\/p>\n

OBJECTIVE ENDPOINTS<\/b><\/p>\n

\u00b7 Erythema index: reflectance colorimetry<\/p>\n

\u00b7 TEWL: transepidermal water loss<\/p>\n

\u00b7 Skin blood flow: laser Doppler flowmetry<\/p>\n

WHY THE DESIGN MATTERS<\/b><\/p>\n

The controlled UVB challenge uses objective biophysical measurements in human volunteers rather than self-assessment.<\/p>\n

It evaluates whether the test formulations can help limit redness and preserve barrier function after a defined oxidative challenge.<\/p>\n

Perugini P, et al. Int J Cosmet Sci. 2008;30(2):113\u2013120. doi:10.1111\/j.1468-2494.2007.00416.x<\/p>\n

08 \u00b7 SKINCARE: HUMAN EVIDENCE<\/p>\n

Approximately 35% lower TEWL after UVB exposure<\/b><\/h2>\n

\u201335%<\/b><\/p>\n

TEWL: measurable preservation of barrier function<\/b><\/p>\n

\u201322%<\/b><\/p>\n

Erythema index: reduced visible redness<\/b><\/p>\n

\u201330%<\/b><\/p>\n

Skin blood flow: reduced hyperemic response<\/b><\/p>\n

Approximate mean reductions versus the UVB-exposed control, for oleuropein emulsions and emulgels. Perugini P, et al. Int J Cosmet Sci. 2008;30(2):113\u2013120.<\/p>\n

\"\"<\/p>\n

Transepidermal water loss (TEWL)<\/b><\/p>\n

\"\"<\/p>\n

Erythema index (colorimetry)<\/b><\/p>\n

\"\"<\/p>\n

Blood flow (laser Doppler)<\/b><\/p>\n

Three instrumental endpoints support reduced redness, barrier disruption and vascular response under the tested conditions.<\/p>\n

08 \u00b7 SKINCARE: HUMAN EVIDENCE<\/p>\n

From acute photostress to photoaging care<\/b><\/h2>\n

\"\"<\/p>\n

The controlled UVB study provides a rationale for daily photoaging care. Longer-term human data on an olive leaf extract cream extend the evidence beyond an acute challenge.<\/p>\n

LONGER-TERM HUMAN DATA: OLIVE LEAF EXTRACT CREAM<\/b><\/p>\n

36 female volunteers; twice daily for 2 months:<\/p>\n

\u00b7 TEWL decreased significantly (p = 0.007), with the effect maintained 1 month after discontinuation.<\/p>\n

\u00b7 Skin hydration increased (p = 0.004).<\/p>\n

\u00b7 Wrinkle parameters improved (p < 0.001).<\/p>\n

\"\"<\/p>\n

Visioscan\u00ae skin surface images: baseline (A), 1 month (B), 2 months (C), and 1 month after discontinuation (D).<\/p>\n

Wanitphakdeedecha R, et al. J Cosmet Dermatol. 2020. doi:10.1111\/jocd.13457<\/p>\n

08 \u00b7 SKINCARE: PRECLINICAL EVIDENCE<\/p>\n

Tissue repair and matrix renewal<\/b><\/h2>\n

\"\"<\/p>\n

Van Gieson staining of collagen deposition in healing skin: oleuropein-treated versus control groups. Mehraein F, et al. Cell J. 2014;16(1):25\u201330.<\/p>\n

\"\"<\/p>\n

VEGF protein expression by Western blot: oleuropein group (Tg) versus control group (Cg).<\/p>\n

Increased VEGF supports microvascular supply to repairing tissue and provides a mechanistic link with matrix renewal.<\/p>\n

RESULTS IN AN AGED-SKIN MODEL<\/b><\/p>\n

Wound closure at day 3: 29.0% versus 19.2% in controls.<\/p>\n

At day 7: 55.3% versus 45.3% in controls.<\/p>\n

Re-epithelialization was complete by day 7 in treated skin.<\/b><\/p>\n

Collagen deposition increased; VEGF increased (p < 0.05).<\/b><\/p>\n

Patent support: EP2570124A1 covers oleuropein compositions for repair of aged and fragile skin.<\/p>\n

Intradermal model in aged mice: preclinical evidence, not a human efficacy claim.<\/p>\n

08 \u00b7 SKINCARE: HUMAN AND IN VITRO EVIDENCE<\/p>\n

Even skin tone: observations in melanin biology<\/b><\/h2>\n

\"\"<\/p>\n

Melanin content in primary human melanocytes (in vitro).<\/b><\/p>\n

\"\"<\/p>\n

Intracellular glutathione (in vitro).<\/b><\/p>\n

\"\"<\/p>\n

Age-spot melanin index versus baseline (D0) in a 12-week human study.<\/p>\n

\"\"<\/p>\n

Placebo versus test product at week 12.<\/b><\/p>\n

IN VITRO: HUMAN MELANOCYTES<\/b><\/p>\n

Melanin content decreased by up to approximately 65%, while intracellular glutathione increased by 55%, supporting the cellular redox-buffering system.<\/p>\n

HUMAN STUDY: AGE SPOTS<\/b><\/p>\n

In a 12-week double-blind, placebo-controlled study of 12 women aged 46\u201372 years, the age-spot melanin index decreased by 34.8% at week 12 versus baseline, with visible lightening.<\/p>\n

The source connects skin-tone care with the redox rationale used for barrier defense.<\/p>\n

Standardized olive polyphenol extract. Lueder M. Cosmet Sci Technol. 2011.<\/p>\n

09 \u00b7 ORAL CARE<\/p>\n

The mouth: a redox\u2013microbial ecosystem<\/b><\/h2>\n

Biofilms reform within hours, saliva continuously dilutes actives, and soft tissues face daily mechanical, chemical and microbial stress. Oral care therefore requires both suitable chemistry and effective delivery.<\/p>\n

BIOFILM AND PLAQUE<\/b><\/p>\n

Bacterial biofilms drive plaque accumulation and acid challenges. Daily biofilm control provides an application opportunity for polyphenols.<\/p>\n

GINGIVAL ENVIRONMENT<\/b><\/p>\n

The gingival margin lies at the intersection of microbial and oxidative stress. Redox support and inflammatory modulation provide relevant development pathways.<\/p>\n

SOFT-TISSUE REPAIR<\/b><\/p>\n

Mucosal tissues face daily irritation and discomfort after care procedures. Repair support is a relevant consumer-care concept.<\/p>\n

Three application opportunities share the same polyphenol rationale: antioxidant activity, inflammatory modulation and microbial inhibition.<\/p>\n

09 \u00b7 ORAL CARE: IN VITRO EVIDENCE<\/p>\n

Published activity against key oral bacteria<\/b><\/h2>\n

\"\"<\/p>\n

In vitro data support a biofilm-management concept and do not constitute therapeutic claims.<\/p>\n

Karygianni L, et al. Biomed Res Int. 2014;2014:839019. doi:10.1155\/2014\/839019.<\/p>\n

S. mutans: Golestannejad Z, et al. Dent Res J (Isfahan). 2020;17(3):179\u2013185. doi:10.4103\/1735-3327.284730 (acid production and adhesion: p < 0.001).<\/p>\n

09 \u00b7 ORAL CARE: HUMAN EVIDENCE<\/p>\n

Biofilm management beyond disinfection<\/b><\/h2>\n

PLAQUE REGROWTH: CROSSOVER CONTROLLED STUDY, N = 17<\/b><\/p>\n

36% lower plaque index versus water rinsing<\/b><\/p>\n

After rinsing with a virgin olive oil preparation containing oleuropein, the plaque index was 1.48 \u00b1 0.36 versus 2.30 \u00b1 0.39 with water (p < 0.001), measured in a plaque-regrowth crossover design.<\/p>\n

Volunteers suspended oral hygiene and underwent objective plaque-regrowth scoring, providing a direct measure of biofilm management under the study conditions.<\/p>\n

Math MV, et al. Int J Dent Health Sci. 2017;4(4).<\/b><\/p>\n

THE POLYPHENOL RATIONALE FOR BIOFILM CARE<\/b><\/p>\n

INTERFERING WITH ADHESION<\/b><\/p>\n

Olive leaf polyphenols reduce S. mutans surface adhesion (p < 0.001).<\/p>\n

MODULATING ACID PRODUCTION<\/b><\/p>\n

Reduced acid production by cariogenic bacteria supports a daily-care rationale relevant to enamel.<\/p>\n

REDOX SUPPORT AT THE GINGIVAL MARGIN<\/b><\/p>\n

Antioxidant activity at the biofilm\u2013gingiva interface provides a rationale for comfort-oriented care.<\/p>\n

Golestannejad Z, et al. Dent Res J (Isfahan). 2020;17(3):179\u2013185.<\/p>\n

09 \u00b7 ORAL CARE: CLINICAL AND PRECLINICAL EVIDENCE<\/p>\n

Oral soft-tissue defense: cytokines and comfort<\/b><\/h2>\n

\"\"<\/p>\n

Mucosal histopathology scores over time: oleuropein versus controls in a mucositis model.<\/p>\n

\"\"<\/p>\n

Tissue MDA, a marker of oxidative damage: oleuropein versus controls (reported p = 0.000).<\/p>\n

CLINICAL: RANDOMIZED CROSSOVER TRIAL, 25 PATIENTS<\/b><\/p>\n

Olive leaf extract mouthwash reduced salivary IL-1\u03b2 and TNF-\u03b1 and was associated with lower mucositis severity scores in chemotherapy patients.<\/p>\n

PRECLINICAL: MUCOSITIS MODEL<\/b><\/p>\n

Oleuropein ointment reduced histopathology scores and tissue MDA (reported p = 0.000), indicating less tissue damage and oxidative stress in the experimental mucosal challenge.<\/p>\n

Positioning: supportive oral care and comfort. Use adjunctive wording rather than therapeutic claims.<\/p>\n

Ahmed KM. Saudi Dent J. 2013;25(4):141\u2013147. doi:10.1016\/j.sdentj.2013.09.001.<\/p>\n

Showraki N, et al. J Dent (Shiraz). 2016;17(4):334\u2013342.<\/p>\n

09 \u00b7 ORAL CARE: DELIVERY SCIENCE<\/p>\n

Why delivery matters in oral care<\/b><\/h2>\n

FOUR ORAL-ENVIRONMENT CHALLENGES<\/b><\/p>\n

Continuous dilution: saliva washes actives away.<\/b><\/p>\n

Short contact time: rinsing lasts minutes rather than hours.<\/p>\n

Oxidative exposure: polyphenols are vulnerable to degradation in aqueous systems.<\/p>\n

Dosage-form constraints: clear rinses, gels and pastes have different formulation requirements.<\/p>\n

THE PURISOME\u00ae APPROACH<\/b><\/p>\n

\u00b7 Vesicles protect polyphenols within the formulation.<\/p>\n

\u00b7 Controlled release aims to extend availability after use.<\/p>\n

\u00b7 The dispersion format suits rinses, gels and pastes.<\/p>\n

SUPPORTING LITERATURE<\/b><\/p>\n

Liposomal encapsulation of olive leaf polyphenols preserved, and under some conditions enhanced, antibacterial activity against S. aureus, including MRSA (MIC 140\u2013240 \u00b5g\/mL). Vesicles remained stable for up to 90 days at 4 \u00b0C.<\/p>\n

Prevete G, et al. ACS Appl Mater Interfaces. 2024;16(50):68850\u201368863. doi:10.1021\/acsami.4c13302<\/p>\n

10 \u00b7 HAIR AND SCALP CARE<\/p>\n

The scalp: a stressed follicular environment<\/b><\/h2>\n

The scalp is skin with approximately 100,000 follicles, abundant sebum and direct UV exposure, creating a concentrated set of environmental challenges.<\/p>\n

PHOTOSTRESS<\/b><\/p>\n

UV exposure is particularly relevant at partings and areas of thinning hair.<\/p>\n

SEBUM OXIDATION<\/b><\/p>\n

Lipid peroxidation at follicular openings increases the oxidative load.<\/p>\n

MICROBIAL PRESSURE<\/b><\/p>\n

A dense, sebum-supported microbial community contributes to ongoing surface challenges.<\/p>\n

FOLLICULAR CYCLE<\/b><\/p>\n

Transition into anagen is sensitive to oxidative and inflammatory signaling.<\/p>\n

Scalp care can draw on the same defensive mechanisms relevant to skin care.<\/p>\n

10 \u00b7 HAIR AND SCALP CARE<\/p>\n

Oleuropein as a rationale for scalp resilience<\/b><\/h2>\n

REDOX DEFENSE<\/b><\/p>\n

Radical scavenging at the scalp surface, where sebum oxidation and UV exposure converge.<\/p>\n

Supporting evidence: in vitro and human skin data.<\/b><\/p>\n

INFLAMMATORY MODULATION<\/b><\/p>\n

A rationale for perifollicular comfort through modulation of inflammatory mediators.<\/p>\n

Supporting evidence: mechanistic studies and human oral-care data.<\/p>\n

MICROBIAL BALANCE<\/b><\/p>\n

Published antibacterial activity against biofilm-forming organisms.<\/p>\n

Supporting evidence: in vitro MIC data.<\/b><\/p>\n

QUALITY CONTROL<\/b><\/p>\n

Autophagy support as a concept for rapidly dividing cells in the follicular environment.<\/p>\n

Supporting evidence: mechanistic literature.<\/b><\/p>\n

FOLLICULAR SIGNALING<\/b><\/p>\n

Wnt\/\u03b2-catenin activation in dermal papilla cells, detailed below.<\/p>\n

Supporting evidence: preclinical data.<\/b><\/p>\n

Each scalp-care pillar is presented with its supporting evidence type.<\/p>\n

10 \u00b7 HAIR AND SCALP: PRECLINICAL EVIDENCE<\/p>\n

Follicular biology: Wnt\/\u03b2-catenin activation<\/b><\/h2>\n

\"\"<\/p>\n

Nanovesicle enrichment through the follicular route: a PURISOME\u00ae delivery concept.<\/p>\n

HUMAN DERMAL PAPILLA CELLS: IN VITRO<\/b><\/p>\n

Oleuropein promoted dermal papilla cell proliferation, increased LEF1 and Cyclin D1, and drove nuclear \u03b2-catenin accumulation, consistent with Wnt pathway activation.<\/p>\n

TELOGEN MOUSE MODEL: TOPICAL APPLICATION FOR 28 DAYS<\/b><\/p>\n

Anagen entry accelerated, with larger and more numerous follicles. Wnt10b, FzdR1, LRP5, IGF-1, KGF, HGF and VEGF increased in skin tissue.<\/p>\n

Evidence level: preclinical. These findings support scalp-care positioning and further development, not a human hair-growth claim.<\/p>\n

Tong T, Kim N, Park T. PLoS ONE. 2015;10(6):e0129578. doi:10.1371\/journal.pone.0129578<\/p>\n

11 \u00b7 APPLICATIONS<\/p>\n

Three application areas, one technology platform<\/b><\/h2>\n

SKINCARE<\/b><\/p>\n

DEFENSE AND REPAIR<\/b><\/p>\n

\u00b7 Human UVB study: TEWL \u201335%; erythema \u201322%.<\/p>\n

\u00b7 Photoaging care: TEWL p = 0.007; wrinkles p < 0.001.<\/p>\n

\u00b7 Even tone: age-spot melanin index \u201334.8%.<\/p>\n

\u00b7 Tissue repair: preclinical wound models.<\/p>\n

ORAL CARE<\/b><\/p>\n

BIOFILM AND SOFT-TISSUE BALANCE<\/b><\/p>\n

\u00b7 Antibacterial activity: published MIC\/MBC values.<\/p>\n

\u00b7 Human crossover study: plaque regrowth \u201336%.<\/p>\n

\u00b7 Clinical rinse study: reduced salivary IL-1\u03b2 and TNF-\u03b1.<\/p>\n

\u00b7 Mucosal comfort: preclinical mucositis model.<\/p>\n

HAIR AND SCALP<\/b><\/p>\n

RESILIENCE AND FOLLICULAR SUPPORT<\/b><\/p>\n

\u00b7 Wnt\/\u03b2-catenin activation: preclinical.<\/p>\n

\u00b7 Follicular delivery: PURISOME\u00ae concept.<\/p>\n

\u00b7 Redox and microbial rationale: evidence from skin and oral studies.<\/p>\n

One ingredient concept connects three care areas through a shared scientific rationale.<\/p>\n

11 \u00b7 APPLICATIONS<\/p>\n

From ingredient to formulation: product concepts<\/b><\/h2>\n

SKINCARE<\/b><\/p>\n

Daily defense serum<\/p>\n

After-sun repair emulgel<\/p>\n

Even-tone concentrate<\/p>\n

Barrier-support lotion<\/p>\n

Informed by human UVB, photoaging and skin-tone studies.<\/b><\/p>\n

ORAL CARE<\/b><\/p>\n

Daily defense mouthwash<\/p>\n

Gingival-care gel<\/p>\n

Biofilm-management toothpaste<\/p>\n

Soothing rinse after care procedures<\/p>\n

Informed by antibacterial, plaque and cytokine evidence.<\/b><\/p>\n

HAIR AND SCALP<\/b><\/p>\n

Pre-wash scalp concentrate<\/p>\n

Leave-on scalp serum<\/p>\n

Clarifying antioxidant shampoo<\/p>\n

Informed by preclinical follicular data and the scalp-stress rationale.<\/p>\n

These are concepts for joint development. Final product claims require testing of the finished formulation.<\/p>\n

11 \u00b7 APPLICATIONS<\/p>\n

NanoActive Oleu™ formulation guidance<\/b><\/h2>\n

HANDLING PRINCIPLES<\/b><\/p>\n

Supplied as an aqueous liposomal dispersion, with no reconstitution required.<\/p>\n

Add during cool-down. Avoid prolonged high shear to protect vesicle integrity.<\/p>\n

Suitable formulation areas include emulsions, serums, gels and oral-care systems.<\/p>\n

Use mild temperatures and pH conditions; validate the preservative system in the complete formulation.<\/p>\n

Detailed specifications, recommended use levels and starter formulations are available from PuriPharm technical services.<\/p>\n

WORKING WITH PURIPHARM<\/b><\/p>\n

Ingredient supply with joint development support.<\/b><\/p>\n

PuriPharm provides application expertise, formulation guidance and supporting evidence documentation, from the first prototype to market-registration materials.<\/p>\n

Contact PuriPharm Co., Ltd. for technical services and joint development.<\/p>\n

NANOACTIVE OLEU™ LIPOSOMAL OLEUROPEIN<\/p>\n

Protect \u00b7 Balance \u00b7 Renew<\/b><\/h2>\n

PHYTOCHEMISTRY: oleuropein-rich Mediterranean polyphenols<\/p>\n

DELIVERY: PURISOME\u00ae FlexVes™ Liposome Technology<\/p>\n

EVIDENCE: published human, in vivo and mechanistic studies<\/p>\n

PARTNERSHIP: joint development with PuriPharm Co., Ltd.<\/p>\n

NanoActive Oleu™<\/p>\n

Powered by PURISOME\u00ae FlexVes™ Liposome Technology<\/p>\n

PuriPharm Co., Ltd.<\/p>\n

REFERENCES<\/p>\n

Key references<\/b><\/h2>\n

01 Perugini P, et al. Lenitive effect of oleuropein on UVB-induced erythema. Int J Cosmet Sci. 2008;30(2):113\u2013120. doi:10.1111\/j.1468-2494.2007.00416.x<\/p>\n

02 Nediani C, et al. Oleuropein, autophagy and oxidative stress\u2013inflammation interplay. Antioxidants. 2019;8(12):578. doi:10.3390\/antiox8120578<\/p>\n

03 Wanitphakdeedecha R, et al. Olive leaf extract cream: TEWL, hydration and wrinkle outcomes. J Cosmet Dermatol. 2020. doi:10.1111\/jocd.13457<\/p>\n

04 Mehraein F, et al. Oleuropein and skin tissue recovery in an aged-mouse model. Cell J. 2014;16(1):25\u201330.<\/p>\n

05 Lueder M. Standardized olive polyphenol extract: melanin, glutathione and age-spot studies. Cosmet Sci Technol. 2011.<\/p>\n

06 Karygianni L, et al. Olive leaf extract against oral pathogens. Biomed Res Int. 2014;2014:839019. doi:10.1155\/2014\/839019<\/p>\n

07 Golestannejad Z, et al. Olive leaf extract vs Streptococcus mutans. Dent Res J (Isfahan). 2020;17(3):179\u2013185. doi:10.4103\/1735-3327.284730<\/p>\n

08 Math MV, et al. Plaque regrowth crossover with virgin olive oil rinse. Int J Dent Health Sci. 2017;4(4).<\/p>\n

09 Ahmed KM. Olive leaf extract mouth rinse: IL-1\u03b2, TNF-\u03b1 and mucositis severity. Saudi Dent J. 2013;25(4):141\u2013147. doi:10.1016\/j.sdentj.2013.09.001<\/p>\n

10 Showraki N, et al. Oleuropein in a chemotherapy-induced mucositis model. J Dent (Shiraz). 2016;17(4):334\u2013342.<\/p>\n

11 Tong T, Kim N, Park T. Oleuropein and hair-follicle Wnt\/\u03b2-catenin signaling. PLoS ONE. 2015;10(6):e0129578. doi:10.1371\/journal.pone.0129578<\/p>\n

12 Prevete G, et al. Liposomal encapsulation of olive leaf polyphenols. ACS Appl Mater Interfaces. 2024;16(50):68850\u201368863. doi:10.1021\/acsami.4c13302<\/p>\n

13 Omar SH. Oleuropein in olive and its pharmacological effects. Sci Pharm. 2010;78(2):133\u2013154.<\/p>\n

14 EP2570124A1. Oleuropein compositions for healing wounds and ulcers in elderly people and\/or diabetics.<\/p>\n

These references concern oleuropein or olive leaf preparations in published studies and patent documents. Evidence types are identified in the relevant sections. PURISOME\u00ae, FlexVes™, NanoActive™ and Oleu™ are trademarks of PuriPharm Co., Ltd.<\/p>\n","protected":false},"excerpt":{"rendered":"

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