PURIPHARM CO. LTD.

NanoActive™ Xantho

The Multi-Pathway Hop Bioactive for Skin & Scalp Longevity

INCI NAME — HUMULUS LUPULUS EXTRACT

Powered by PURISOME® Liposome Delivery Technology

XANTHOHUMOL · C21H22O5 · A PRENYLATED CHALCONE FROM HUMULUS LUPULUS

02 · POSITIONING

More Than a Botanical Antioxidant

From Hops to High-Performance Beauty Science

Xanthohumol is a prenylated chalcone naturally present in hops (Humulus lupulus L.) that connects several major biological pathways underlying visible skin aging, uneven tone, redness, blemish-prone skin and scalp stress.

NanoActive™ Xantho brings this multi-pathway biology into modern cosmetic formulation through advanced liposome delivery.

01｜Oxidative Defense:multi-ROS and singlet-oxygen quenching capacity

02｜Inflammation Control:modulation of NF-κB-driven inflammatory signaling

03｜Tone Optimization:keratinocyte–melanocyte stress communication

04｜Matrix Preservation:MMP and elastase inhibition, structural protein support

05｜Blemish Ecosystem Support:microbial, oxidative and inflammatory balance

06｜Scalp Homeostasis:a healthier foundation for scalp and hair

03 · PRODUCT PROFILE

NanoActive™ Xantho at a Glance

A Clean Technical Profile — Confirmed Information Only

Item：Product

Confirmed information：NanoActive™ Xantho

Item：INCI name

Confirmed information：HUMULUS LUPULUS EXTRACT

Item：Botanical source

Confirmed information：Humulus lupulus L.

Item：Signature bioactive

Confirmed information：Xanthohumol

Item：Bioactive family

Confirmed information：Prenylated chalcones

Item：Technology platform

Confirmed information：NanoActive™ · PURISOME® Liposome Delivery

Item：Application fields

Confirmed information：Skin Care + Scalp & Hair Care

Core Cosmetic Positioning

Antioxidant · Soothing · Tone-Evening · Anti-Aging · Blemish Care · Scalp Care

One botanical molecule. Multiple interconnected pathways of skin and scalp aging.

04 · THE MOLECULE

Xanthohumol: A Distinctive Prenylated Chalcone

Botanical Origin. Molecular Precision.

Xanthohumol combines the radical-scavenging chemistry of a polyphenol with a prenylated molecular architecture that supports interactions with lipid-rich biological environments.

Prenyl side chain: affinity for lipid-rich biological environments. Polyphenolic chalcone core: radical-scavenging chemistry.

Humulus lupulus L.: the cone of the hop plant, a natural reservoir of prenylated chalcones.

Reported Biological Activities

Reported biological activity：Antioxidant

Description：Multi-ROS defense

Reported biological activity：Antimicrobial

Description：Broad Gram-positive activity

Reported biological activity：Anti-inflammatory

Description：NF-κB pathway modulation

Reported biological activity：Pigmentation-modulating

Description：Multi-level tone pathways

Reported biological activity：ECM-protective

Description：MMP and elastase inhibition

Kołodziejczak A, et al. Int J Mol Sci. 2024;25:11938. · Oledzka E. Int J Mol Sci. 2024;25:3398.

05 · DELIVERY SCIENCE

Why Advanced Delivery Matters

Great biology meets a formulation challenge: NanoActive™ technology translates a lipophilic botanical active into a formulation-friendly format.

PURISOME® liposome concept—xanthohumol positioned within the phospholipid bilayer

Native xanthohumol：Highly lipophilic chalcone

NanoActive™ Xantho：Liposome-based incorporation strategy

Native xanthohumol：Poor aqueous solubility

NanoActive™ Xantho：Improved dispersion concept

Native xanthohumol：Challenging dispersion in water-rich cosmetic systems

NanoActive™ Xantho：Protection within a lipid-compatible microenvironment

Native xanthohumol：Delivery depends strongly on formulation environment

NanoActive™ Xantho：Designed for modern skin and scalp formulations

Native xanthohumol：—

NanoActive™ Xantho：A rational route to unlock a hydrophobic botanical active

Published research has investigated xanthohumol delivery using nanoliposomes, nanoparticles, solid lipid nanoparticles, microemulsions and other nanocarrier systems. Liposome-based delivery is a rational formulation strategy for sophisticated skin and scalp care systems.

Oledzka E. Int J Mol Sci. 2024;25:3398. · Khatib N, et al. J Food Process Preserv. 2019;43:e14075. · Harish V, et al. Pharmaceutics. 2022;14:2403.

06 · MECHANISM MAP

One Active, Multiple Beauty Pathways

A Systems Approach to Skin & Scalp Longevity

A systems approach to skin and scalp longevity

Pathway：ROS / singlet oxygen

Reported direction：Quenched ↓

Pathway：NF-κB inflammatory signaling

Reported direction：Modulated ↓

Pathway：GM-CSF / melanocyte activation

Reported direction：Suppressed ↓

Pathway：MMPs + elastase

Reported direction：Inhibited ↓

Pathway：Collagen · elastin · fibrillin

Reported direction：Supported ↑

Pathway：Acne-associated microbial pressure

Reported direction：Reduced ↓

Visible Outcomes

More resilient-looking skin · reduced appearance of redness · more even-looking tone · firmer, smoother appearance · blemish-prone skin support · healthier scalp environment.

07 · ANTIOXIDANT EVIDENCE

Antioxidant Performance Beyond Conventional Benchmarks

Exceptional Defense Against Multiple Reactive Oxygen Species

Total ORAC — Published Assay

Total ORAC: xanthohumol 4.20, green tea catechin reference 4.20, vitamin C 1.40, vitamin E 0.75

Xanthohumol delivered approximately 3× the total ORAC value of vitamin C and more than 5× that of vitamin E in this assay system.

Singlet Oxygen Absorbance Capacity — SOAC

SOAC sample：Xanthohumol

Vitamin E equivalents：14.1

SOAC sample：Green tea catechin reference

Vitamin E equivalents：1.8

SOAC sample：Vitamin E

Vitamin E equivalents：1.0

Why Singlet Oxygen Matters

Lipid oxidation · squalene peroxidation · photo-oxidative stress · inflammatory amplification · visible aging.

Yamaguchi N, Satoh-Yamaguchi K, Ono M. Phytomedicine. 2009;16:369–376. DOI: 10.1016/j.phymed.2008.12.021.

08 · INFLAMMATION CONTROL

Breaking the Oxidative–Inflammatory Loop

Calm the Signals That Accelerate Visible Aging

Xanthohumol—Interrupts the Cascade

Environmental / UV stress→ROS generation→TLR4 / MD2—NF-κB activation

→TNF-α · IL-1β · IL-6 · IL-12 · NO→Redness · irritation · ECM degradation · uneven appearance

Published Evidence

Suppression of NF-κB-related inflammatory signaling; reduced IL-12 production; reduced nitric oxide production; modulation of TNF-α, IL-1β and IL-6 signaling; and reduced chronic inflammatory response in experimental skin models.

Designed to help move stressed skin from inflammatory reactivity toward visible equilibrium.

Cho Y-C, et al. Int Immunopharmacol. 2010;10:556–561. · Philips N, et al. Anti-Inflamm Anti-Allergy Agents Med Chem. 2010;9:142–149. · Kołodziejczak A, et al. Int J Mol Sci. 2024;25:11938.

09 · TONE SCIENCE｜GM-CSF

Interrupting Keratinocyte-to-Melanocyte Stress Signaling

GM-CSF—A Distinctive Skin-Stress Target

UV and chemical stress activate epidermal keratinocytes→Stressed keratinocytes release GM-CSF→Melanocyte activation and stress-associated pigmentation signaling

Reduction of Stress-Induced GM-CSF Release—In Vitro

PMA-induced stress −48%; UVB-induced stress −21%

Stress model：PMA-induced stress

With xanthohumol：52% of control, a 48% reduction

Positive-control context：57% reduction

Stress model：UVB-induced stress

With xanthohumol：79% of control, a 21% reduction

Positive-control context：45% reduction

Targeting the communication between stressed keratinocytes and melanocytes.

Patent-reported keratinocyte data: WO2010044076.

10 · TONE SCIENCE｜EX VIVO

Ex Vivo Human Skin: Keeping the UV Stress Signal Near Baseline

A Compelling Keratinocyte–Melanocyte Story

Study element：Model

Detail：Human skin explants

Study element：Donor panel

Detail：n=6 donors

Study element：Skin phototype

Detail：Phototype III

Study element：Stress induction

Detail：UVA + UVB challenge

Active melanocytes: baseline 5.6, UVAB 10.5, UVAB + xanthohumol approximately basal

Endpoint：GM-CSF release

Baseline：5.35 pg/mL

UVAB：approximately 4× baseline

UVAB + xanthohumol：6.95 pg/mL

Endpoint：Active melanocytes

Baseline：5.6

UVAB：10.5

UVAB + xanthohumol：approximately basal

Xanthohumol helped disconnect UV stress from melanocyte activation.

Patent-reported ex vivo human skin data: WO2010044076.

11 · TONE SCIENCE｜MULTI-LEVEL

More Than Tyrosinase: Multi-Level Tone Optimization

Target Pigmentation Across the Epidermal Signaling Network

01｜Signal—GM-CSF stress-signal control:reduced keratinocyte-driven melanocyte activation following UV stress

02｜Synthesis—melanogenic enzyme modulation:published mushroom tyrosinase studies demonstrate potent inhibition of L-tyrosine and L-DOPA oxidation

03｜Transfer—reduced melanocyte dendricity:reported decrease in dendrite number and length, potentially reducing pigment transfer

04｜Clearance—melanosome clearance:published cell studies indicate enhanced melanosome degradation in keratinocytes

From pigment signaling to pigment transfer and clearance—a multi-level approach to visible tone uniformity.

Goenka S, Simon SR. Biochem Biophys Rep. 2021;26:100955. · Kołodziejczak A, et al. Int J Mol Sci. 2024;25:11938. · WO2010044076.

12 · HUMAN EVIDENCE

Human Use Data: Visible Tone Evening in 8 Weeks

Patent-Reported Human Cosmetic Study

Study Design

25 female subjects; mean age 34.1 ± 6.1 years; phototypes III–IV; twice-daily application for 8 weeks; test cream containing 3% P1 xanthohumol preparation, equivalent to 22.5 ppm xanthohumol. Measurements included L*, ITA°, redness a* and pigmented-spot morphology.

Result：Objective brightness / ITA° criteria

Finding：68–72% of subjects demonstrated improvement after 8 weeks

Result：Pigmented spot area

Finding：−12.6% after 8 weeks

Result：Visible redness

Finding：Significantly reduced after 8 weeks

Visible tone uniformity backed by human cosmetic-use data.

Patent-reported human-use data: WO2010044076. Not proprietary NanoActive™ Xantho clinical testing.

13 · MATRIX LONGEVITY

Defending the Youthful Extracellular Matrix

Protect What Gives Skin Its Strength and Elasticity

Matrix Synthesis ⇌ Matrix Degradation

Slows degradation：Inhibits MMP-1, MMP-2, MMP-8, MMP-9 and elastase

Supports structural renewal：Published fibroblast data report increased biosynthesis of collagen I, III and V, elastin, fibrillin-1 and fibrillin-2

Slows degradation：MMP-1 inhibition: 91% at 100 µg/mL MMP-8 inhibition: 99% at 100 µg/mL

Supports structural renewal：Structural proteins associated with skin strength and elasticity

Slows degradation：Reported IC50: MMP-1 20.5 µg/mL; MMP-8 16.8 µg/mL

Supports structural renewal：—

Dual-action matrix strategy—slow degradation while supporting structural renewal.

Yamaguchi N, et al. Phytomedicine. 2009;16:369–376. · Philips N, et al. J Cosmet Sci. 2010;61:125–132.

14 · BLEMISH CARE

A Multi-Target Active for Blemish-Prone Skin

Beyond Antibacterial: Rebalancing the Blemish Ecosystem

Target：Microbial pressure

Direction：Acne-associated bacteria ↓

Target：Oxidative stress

Direction：ROS and singlet oxygen ↓

Target：Inflammatory signaling

Direction：COX-2 / PGE2 / IL-1β / TNF-α-associated pathways ↓

Target：Matrix stress

Direction：MMP-driven collagen degradation ↓

Published MIC Values—µg/mL

MIC values: C. acnes 3 µg/mL; S. aureus 1; S. epidermidis 3; S. pyogenes 1

Organism：C. acnes

Published MIC, µg/mL：3

Organism：S. aureus

Published MIC, µg/mL：1

Organism：S. epidermidis

Published MIC, µg/mL：3

Organism：S. pyogenes

Published MIC, µg/mL：1

Cutibacterium acnes was reported as Propionibacterium acnes in the original study.

Antioxidant + antimicrobial + inflammation-modulating + matrix-protective activity in one botanical platform.

Yamaguchi N, et al. Phytomedicine. 2009;16:369–376.

15 · SCALP & HAIR CARE

Scalp-First Hair Care

Healthy-Looking Hair Starts with Scalp Homeostasis

Scalp and follicular microenvironment—oxidative, sebum and microbial stress signals

01｜Oxidative-Stress Defense:helps counter environmental and photo-oxidative stress affecting the scalp

02｜Scalp Comfort:supports modulation of inflammatory signaling associated with visible redness and discomfort

03｜Sebum Environment Support:hop and xanthohumol literature supports sebum-conscious cosmetic concepts

04｜Microbial-Balance-Oriented Care:broad activity against relevant Gram-positive microorganisms supports scalp-purifying concepts

05｜Follicular Environment Protection:helps maintain a healthier biochemical environment around the scalp and follicular opening

A scalp-first active for stronger skin foundations and healthier-looking hair.

WO2010044076 describes topical xanthohumol applications for improving the overall condition of skin and scalp.

16 · APPLICATIONS

Formulation & Application Opportunities

From High-Performance Facial Care to Advanced Scalp Systems

Skin Care：Antioxidant serums; tone-evening essences; anti-aging concentrates

Scalp & Hair Care：Scalp serums; scalp tonics; scalp essences

Skin Care：Blemish-prone skin serums; post-stress soothing products; redness-care formulations

Scalp & Hair Care：Sebum-conscious scalp care; sensitive-scalp formulas; anti-pollution scalp care

Skin Care：Urban defense / anti-pollution care; after-sun skin care

Scalp & Hair Care：Premium shampoos; conditioners; leave-on scalp treatments

Skin Care：Eye and facial care; premium night-repair products

Scalp & Hair Care：Hair-density appearance support concepts*

*Based on scalp health.

Suitable for development across sophisticated leave-on and rinse-off skin and scalp formats.

17 · THE NANOACTIVE™ XANTHO ADVANTAGE

One Active. Six Strategic Beauty Territories.

01｜Antioxidant Shield:multi-ROS and singlet-oxygen defense

02｜Redness & Comfort:multi-pathway inflammatory-signaling modulation

03｜Even-Tone Science:GM-CSF, melanocyte activation, pigment transport and melanosome pathways

04｜Matrix Longevity:MMP / elastase inhibition plus structural-protein support

05｜Blemish Ecosystem:antimicrobial, antioxidant, inflammatory and matrix approach

06｜Scalp Homeostasis:oxidative, inflammatory, sebum and microbial-environment support

Multi-Pathway Botanical Intelligence, Delivered by Liposome Science.

NanoActive™ Xantho transforms the biological versatility of hop-derived xanthohumol into a modern active platform for next-generation skin and scalp care.

18 · EVIDENCE BASE

Selected Scientific References

Human & Ex Vivo Evidence · Primary Mechanistic Data · High-Quality Reviews

01｜Yamaguchi N, Satoh-Yamaguchi K, Ono M. In vitro evaluation of antibacterial, anticollagenase, and antioxidant activities of hop components addressing acne vulgaris.Phytomedicine.2009;16:369–376. DOI: 10.1016/j.phymed.2008.12.021.

02｜Philips N, Samuel M, Arena R, et al. Direct inhibition of elastase and matrix metalloproteinases and stimulation of biosynthesis of fibrillar collagens, elastin, and fibrillins by xanthohumol.J Cosmet Sci.2010;61:125–132.

03｜Cho YC, You SK, Kim HJ, et al. Xanthohumol inhibits IL-12 production and reduces chronic allergic contact dermatitis.Int Immunopharmacol.2010;10:556–561.

04｜Goenka S, Simon SR. Depigmenting effect of xanthohumol from hop extract in MNT-1 human melanoma cells and normal human melanocytes.Biochem Biophys Rep.2021;26:100955.

05｜Kołodziejczak A, Dziedzic M, Algiert-Zielińska B, et al. A Novel Look at Mechanisms and Applications of Xanthohumol in Dermatology and Cosmetology.Int J Mol Sci.2024;25:11938. DOI: 10.3390/ijms252211938.

06｜Oledzka E. Xanthohumol—A Miracle Molecule with Biological Activities: A Review of Biodegradable Polymeric Carriers and Naturally Derived Compounds for Its Delivery.Int J Mol Sci.2024;25:3398. DOI: 10.3390/ijms25063398.

07｜Gerhäuser C. Broad spectrum antiinfective potential of xanthohumol from hop in comparison with activities of other hop constituents and metabolites.Mol Nutr Food Res.2005;49:827–831.

08｜WO2010044076. Cosmetic and topical uses of xanthohumol, including skin brightening, reduction of skin redness and GM-CSF-related mechanisms.

Evidence hierarchy: human / ex vivo cosmetic evidence → primary peer-reviewed mechanistic data → high-quality scientific reviews.

P U R I P H A R M   C O . ,   L T D .

PURIACTIVES® · NANOACTIVE™ Nanoliposome Series

NanoActive™ GalaBiome™

Nanoliposomal Prebiotic Barrier Platform

Microbiome × Hydration × Barrier

PuriPharm Co. Ltd.

 

PARADIGM SHIFT

Moisturization Is More Than Adding Water

Skin hydration is an ecosystem function.

Traditional moisturization focuses on humectants, occlusives, and emollients. Modern skin science shows that skin hydration is an ecosystem function created by multiple interacting factors.

SIX KEY FACTORS

Lipid architecture; natural moisturizing factor (NMF); tight junctions; water transport; the acid mantle; and the skin microbiome.

A MUTUALLY SUPPORTIVE LOOP

Healthy microbiome ↕ healthy barrier ↕ healthy hydration

The three dimensions reinforce one another. An imbalance in any one dimension can propagate through the whole system—this is the scientific basis of “ecosystem hydration.”

Reference: Lee HJ, Kim M. Skin Barrier Function and the Microbiome. Int J Mol Sci. 2022;23:13071. doi:10.3390/ijms232113071.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

MICROBIOME × BARRIER

Microbiome & Barrier: A Bidirectional Symbiotic Defense Network

DEFENSE PATHWAY

Microbial barrier → acid mantle → metabolites/innate signals → keratinocyte differentiation → tight junctions → ceramide lipid barrier

TEWL ↓ · HYDRATION ↑

Lower transepidermal water loss and improved skin hydration

Healthy commensal microorganisms help build four layers of defense:

PHYSICAL

Commensals occupy ecological niches and reinforce the integrity of surface defenses.

CHEMICAL

Metabolism produces organic acids that help maintain the acid mantle.

MICROBIAL

Competition for nutrients and adhesion sites limits the dominance of opportunistic microbes.

IMMUNE

Contributes to homeostatic regulation of innate and adaptive immune signaling.

Healthy commensals do more than simply exist on the skin surface; they actively participate in the skin’s defense network.

Reference: Lee HJ, Kim M. Skin Barrier Function and the Microbiome. Int J Mol Sci. 2022;23:13071. doi:10.3390/ijms232113071.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

PRODUCT OVERVIEW

One Platform. Four Biological Dimensions.

SELECTIVE PREBIOTIC POLYOL

Lactitol: a lactose-derived disaccharide polyol that supports a healthy skin microbiome through selective prebiotic logic—selective nourishment rather than broad-spectrum feeding.

HYDRATION & BARRIER BIOLOGY

XAX ternary sugar-derivative system: Xylitylglucoside + Anhydroxylitol + Xylitol—multi-pathway hydration biology spanning water reserves, water circulation, and barrier lipids.

BIOMIMETIC LIPID INTERFACE

Phospholipids: amphiphilic structures analogous to biological membranes, creating a biomimetic lipid interface while supporting barrier lipids.

ADVANCED DELIVERY ARCHITECTURE

NanoActive™ nanoliposomes: nanoscale phospholipid vesicles encapsulate the aqueous phase of sugar-based actives for uniform distribution, local retention, and synergistic delivery.

Biome × hydration × barrier × delivery—one platform, four biological dimensions.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

LACTITOL

Lactitol | Selective Prebiotic Logic from Sugar Science

IDENTITY

Lactitol is a lactose-derived disaccharide polyol. Its value is not limited to humectancy; it lies in the prebiotic potential created by selective fermentation.

Feed the beneficial ecosystem, rather than simply feeding everything.

SCIENTIFIC EVIDENCE

Pure-culture screening compared how broadly different candidate prebiotic substrates were used by microorganisms:

• Lactitol was used with high specificity by Lactobacillus-related strains;
• Common substrates such as galacto-oligosaccharides and fructo-oligosaccharides were used by a broader range of microorganisms;
• The study reported that this degree of selectivity had not been observed among previously established prebiotics.

This selective utilization profile makes lactitol an ideal sugar-based nutritional substrate for targeted support of beneficial microbiota.

In-vitro pure-culture evidence for lactitol’s prebiotic mechanism supports its selective nourishment rationale.

Reference: Mäkeläinen H, Saarinen M, Stowell J, Rautonen N, Ouwehand AC. Beneficial Microbes. 2010;1(2):139-148. doi:10.3920/BM2009.0029.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

SELECTIVE ECOLOGY

Lactitol + Xylitol: Selective Skin Ecology

Selective polyol ecology—not “killing microbes,” but shaping ecological selection.

ECOLOGICAL SELECTION

The polyol combination provides selectively utilized nutritional substrates.

SUPPORT HEALTHY RESIDENT MICROBIOTA

Resident commensals gain a nutritional advantage.

LIMIT OPPORTUNISTIC DOMINANCE

Helps restore a more stable skin ecosystem.

In-vitro evidence

30–50%

In-vitro inhibition of growth for selected opportunistic microorganisms by the polyol system

≈100%

Increase in growth for selected healthy resident skin microorganisms

Suppress opportunistic advantage while supporting resident commensals—this is the essential difference between ecological selection and broad-spectrum antimicrobial strategies.

Reference: WO1995015149A1. Novel Cosmetic Compositions Containing Simple Polyols. 1995.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

ACID MANTLE

Supporting the Skin’s Acidic Ecosystem

The skin’s acidic environment is both a chemical barrier and a driver of microbial selection. A cleansing-induced rise in pH temporarily disrupts this balance; a polyol ecosystem-support system can help the skin return more quickly to its physiological acidic state.

Human study: 2% lactitol + 2% xylitol system

TIMELINE

Washing → pH rises and the acid mantle is temporarily disrupted → polyol ecosystem support → faster return to physiological acidic pH

4–6 h

Typical time required for the cleansing-induced pH rise to return to baseline

≈3 h

Recovery time after use of the polyol system (p < 0.05), with a more favorable microbial composition also observed

Reference: WO1995015149A1. Novel Cosmetic Compositions Containing Simple Polyols. 1995.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

XAX SYSTEM

XAX | From Humectancy to Hydration Biology

XAX = Xylitylglucoside + Anhydroxylitol + Xylitol. It is not an ordinary sugar-based humectant, but a hydration system acting simultaneously on three biological axes:

AXIS 01 · WATER RESERVOIR

Enhances water-reserve systems such as hyaluronic acid and chondroitin sulfate, building an internal skin “reservoir.”

AXIS 02 · BARRIER LIPIDS

Promotes ceramide-related synthesis and optimization of the lipid barrier, reducing water loss at its source.

AXIS 03 · WATER CIRCULATION

Modulates mechanisms related to tight junctions and epidermal water transport, enabling orderly movement of water through the skin.

Reservoir + lipids + circulation: from “holding water” to “managing water”—XAX forms the biological hydration backbone of GalaBiome™.

Reference: Garcia C, et al. Asian J Beauty Cosmetol. 2023;21(1):13-27. doi:10.20402/ajbc.2022.0051.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

CERAMIDE BIOLOGY

Reshaping the Lipid Logic of the Stratum Corneum

BRICK & MORTAR MODEL

Corneocytes are the “bricks,” while intercellular lipids such as ceramides form the “mortar.”

MECHANISM

XAX → de novo ceramide synthesis ↑ + cholesterol synthesis pathway (HMGCR-related) ↑ → more orderly intercellular lipid matrix → barrier strength ↑ → TEWL ↓

In-vitro evidence · De novo ceramide synthesis

Human skin explant model: 3% XAX versus untreated control (P < 0.05); differences for placebo and glycerol were not statistically significant (ns). Barrier improvement consistent with reduced TEWL was also observed.

Reference: Garcia C, Valin E, Hernandez E, Kern C, Roso A. Asian J Beauty Cosmetol. 2023;21(1):13-27. doi:10.20402/ajbc.2022.0051.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

WATER RESERVOIR

Building an Internal Water Reservoir in the Skin

MECHANISM

XAX → acts on fibroblasts and keratinocytes → hyaluronic acid (HA) ↑ + chondroitin sulfate (CS) ↑ → extracellular-matrix water-binding capacity ↑ → expansion of the skin’s internal water reservoir

Hyaluronic acid and chondroitin sulfate are core glycosaminoglycans in dermal–epidermal water reserves and determine the skin’s water-storage capacity.

Cell model · Dose-dependent stimulation of chondroitin sulfate synthesis

In-vitro cultured human skin fibroblasts; mechanistic cell-model data. Differences in the two higher-concentration groups were statistically significant (P < 0.05). XAX also increased hyaluronic acid production in the cell model.

Reference: Garcia C, Valin E, Hernandez E, Kern C, Roso A. Asian J Beauty Cosmetol. 2023;21(1):13-27. doi:10.20402/ajbc.2022.0051.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

WATER CIRCULATION

Hydration Is a Dynamic Flow

Hydration is not simply the static act of “holding water.”

HYDRATION FLOW

Reservoir → transport → retention → controlled loss

WATER FLOW PATHWAY

Dermis → epidermis · tight junctions → stratum corneum

XAX-REGULATED GENES

CGN (cingulin); CLDN-4/CLDN-5/CLDN-7 (claudins); and multiple genes related to epidermal differentiation, keratinization, and tight junctions.

Evidence derives from a gene-expression study in a 3D reconstructed epidermis model.

Water reservoir + water circulation + barrier retention—this is the scientific narrative that differentiates GalaBiome™ from a simple humectant.

Reference: Garcia C, Valin E, Hernandez E, Kern C, Roso A. Asian J Beauty Cosmetol. 2023;21(1):13-27. doi:10.20402/ajbc.2022.0051.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

 

 

HUMAN EVIDENCE

Long-Lasting Hydration Beyond the Moment of Application

Human study: 25 female volunteers with dry skin used 3% XAX for 30 days. Benefits progressed over time, moving from immediate hydration toward structural improvement.

8 HOURS

Corneometry +6% and TEWL −8%—an immediately visible hydration response.

DAY 15

Hydration benefits remained stable while barrier indicators continued to improve.

DAY 30

Corneometry +11% and TEWL −15%—a transition from water replenishment to barrier-structure improvement.

Corneometry reflects stratum-corneum hydration, while TEWL was measured by evaporimetry. The same study also observed approximately 33% improvement in skin micro-roughness and approximately 25% improvement in desquamation.

Human research on XAX validates its multi-pathway hydration and barrier mechanisms—GalaBiome™ integrates them into a unified ecosystem platform.

Reference: Garcia C, Valin E, Hernandez E, Kern C, Roso A. Asian J Beauty Cosmetol. 2023;21(1):13-27. doi:10.20402/ajbc.2022.0051.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

RAMAN SCIENCE

Not All Water in the Stratum Corneum Is the Same

GLOBAL WATER

Overall hydration level of the stratum corneum.

PARTIALLY BOUND WATER

Water weakly associated with stratum-corneum structures.

UNBOUND WATER

Freely mobile water molecules.

KEY RAMAN FINDINGS — SUGAR-DERIVED COMPLEX

• All three water states improved: global, partially bound, and unbound water
• Water-retention capacity increased
• Lateral organization of stratum-corneum lipids became more orderly
• Keratin structural stability improved
• Effects were observed not only at the surface but also at a depth of 8 μm

CONFOCAL RAMAN DEPTH PROFILE

Surface: all three water states improved.

At 8 μm in the stratum corneum: improvement was also observed—benefits were not limited to the surface.

Reference: Fluhr JW, Tfayli A, Darlenski R, et al. J Biophotonics. 2023;16:e202200201. doi:10.1002/jbio.202200201.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

NANOLIPOSOME

From Prebiotic Molecules to a Biomimetic Delivery Interface

NANOLIPOSOME STRUCTURE

Aqueous core: an active aqueous pool of lactitol + XAX.

Phospholipid bilayer: surrounds the aqueous core.

External water phase: vesicles are dispersed in the continuous aqueous phase.

WHAT THE SYSTEM PROVIDES

• A reservoir for aqueous actives
• A phospholipid-bilayer interface
• More uniform distribution across the skin/scalp surface
• Synergy with a biomembrane-like lipid environment
• Improved delivery performance and user experience
• A dual microbiome + barrier platform

The phospholipid bilayer has an amphiphilic structure similar to biological membranes—this delivery architecture differentiates GalaBiome™ from an ordinary blended solution.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

DELIVERY DIRECTION

A Scientifically Established Delivery Direction

CARRIER DIRECTIONS ESTABLISHED IN PUBLIC PATENTS

Polyols such as lactitol and xylitol may be delivered in cosmetics as liposomes, micro/nanoparticles, or micro/nanocapsules.

MORE RECENT EXTENSIONS

Public patent research has also explored topical delivery of microbiome-related actives using phospholipid liposomes. Liposomes × microbiome care is a scientifically validated technical direction.

PATENT REFERENCES

WO1995015149A1 (1995) | Novel Cosmetic Compositions Containing Simple Polyols.

US20240216265A1 (2024) | Liposomal Vegan Probiotic Skin Treatment.

GalaBiome™’s polyol × liposome architecture is built on decades of publicly available scientific development.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

16 · INTEGRATED MECHANISM

The GalaBiome™ 4D Mechanism

NanoActive™ GalaBiome™

Balanced microbiome × optimized hydration × stronger barrier × improved skin & scalp comfort

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

POSITIVE FEEDBACK

Microbiome–Ceramide Crosstalk

FORWARD

Healthy microbiome → metabolic activity of commensals such as S. epidermidis → sphingomyelinase participates in host ceramide formation → ceramide homeostasis → barrier integrity + water retention ↑

REVERSE

A healthy, stable barrier with appropriate pH → dense lipid matrix and intact acid mantle → more secure commensal niches → a more stable microbiome

Positive Microbiome–Barrier Loop

Frontier science from skin-microbiome reviews indicates that healthy commensals participate in barrier-lipid homeostasis through metabolic activity. This is the theoretical foundation of GalaBiome™’s integrated microbiome × barrier design.

Reference: Lee HJ, Kim M. Skin Barrier Function and the Microbiome. Int J Mol Sci. 2022;23:13071. doi:10.3390/ijms232113071.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

18 · SCALP ECOSYSTEM

The Scalp Is Skin — With a Different Ecosystem

High sebum × abundant follicles: a distinctive microenvironment.

High microbial density: a more sensitive microbial balance.

Barrier readily affected by cleansing and the environment: more fragile water and lipid homeostasis.

Blow-drying/coloring/perming: thermal and chemical stress.

Environmental stress: dryness, pollution, and UV exposure.

Scalp care cannot focus only on dandruff removal or oil control; three dimensions must be managed together:

Scalp microbiome × scalp barrier × scalp hydration

GalaBiome™ = Scalp Ecosystem Conditioner

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

SCALP EVIDENCE

Scalp Hydration & Barrier Support

Human scalp study of a lactitol/xylitol system

28 volunteers (16 women + 12 men) · 10-day regimen · used in shampoo, conditioner, and leave-on scalp care.

+27%

Immediate moisturization: increase in scalp hydration immediately after application

+17%

Long-lasting moisturization: sustained scalp hydration

BARRIER & DESQUAMATION

TEWL results supported improved scalp-barrier integrity; videomicroscopy showed improvement in the appearance of scalp desquamation.

From hydration to barrier support, and from immediate to lasting benefits, the polyol ecosystem concept also applies to the scalp—providing human evidence for GalaBiome™ scalp applications.

Reference: Human scalp claim substantiation study, 28 volunteers, 10-day regimen, Dec. 2004. · WO2006061678A1. Cosmetic or Dermatological Compositions of Saccharose Substitutes.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

APPLICATIONS

One Biological Platform. Multiple Beauty Applications.

SKIN

• Barrier-repair serum
• Hydrating serum
• Cream for sensitive skin
• Microbiome-supporting moisturizing lotion
• Gel cream/sheet mask
• Gentle cleansing/post-cleansing care

SCALP & HAIR

• Scalp essence/scalp treatment
• Shampoo/conditioner
• Hair mask
• Leave-on scalp treatment
• Anti-dryness scalp care
• Sensitive-scalp care

Healthy scalp → a better environment for hair. Scalp hydration, comfort, and barrier support—combined with the emollient sensory profile and hair-care compatibility of phospholipids—make a healthy scalp the best environment for beautiful hair.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

FOR FORMULATORS

Designed for Modern Formulation Thinking

A single humectant mainly addresses immediate water attraction; a single prebiotic mainly supports a microbiome concept; traditional barrier lipids mainly provide lipid replenishment. GalaBiome™ integrates prebiotic support + hydration biology + barrier support + nanoliposomes into one platform.

Technical dimension	Humectant	Single prebiotic	GalaBiome™
Immediate hydration	✓	—	✓
Biological hydration	—	—	✓
Microbiome support	—	✓	✓
Barrier support	Limited	Indirect	✓
Nanoliposomal delivery	—	—	✓
Skin + scalp	Limited	Possible	✓

 

One addition covers four core dimensions of modern formulation.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

CLAIM ARCHITECTURE

Claim Architecture

MICROBIOME

• Supports skin ecosystem balance
• Helps maintain microbiome homeostasis
• Prebiotic skin and scalp care

HYDRATION

• Immediate and long-lasting moisturization
• Supports skin water reserves
• Optimizes water-retention capacity

BARRIER

• Helps strengthen the moisture barrier
• Supports ceramide and lipid homeostasis
• Helps reduce excessive water loss

SCALP

• Supports scalp hydration
• Improves scalp comfort
• Helps maintain scalp ecosystem balance
• Supports a healthy-looking scalp

TECHNOLOGY

• Nanoliposomal prebiotic platform
• Biomimetic phospholipid delivery
• Microbiome–barrier synergy

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

PRODUCT CONCEPTS

From Ingredient to Product Concepts

MICROBIOME BARRIER SERUM

Prebiotic ecosystem support; ceramide biology; long-lasting hydration.

SKIN ECOSYSTEM CREAM

Barrier strengthening; comfortable skin feel; suitable for dry and sensitive skin.

SCALP BIOME ESSENCE

Scalp hydration; improved appearance of flaking; scalp comfort.

HYDRATING BIOME SHAMPOO

Gentle cleansing; scalp-ecosystem friendly; post-wash comfort.

Four premium concept directions—from face to scalp, multiple expressions of the same platform.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

 

24 · SUMMARY

NanoActive™ GalaBiome™

Microbiome × Hydration × Barrier × Nanoliposome

A Smarter Ecosystem for Skin & Scalp.

PuriPharm Co. Ltd.

 

REFERENCES

Scientific References

JOURNALS

1. Lee HJ, Kim M. Skin Barrier Function and the Microbiome. Int J Mol Sci. 2022;23:13071. doi:10.3390/ijms232113071.
2. Garcia C, Valin E, Hernandez E, Kern C, Roso A. Effect of a Simple Sugar-based Ingredient on Skin Moisturization: Biological Mode of Action and Clinical Effects. Asian J Beauty Cosmetol. 2023;21(1):13-27. doi:10.20402/ajbc.2022.0051.
3. Fluhr JW, Tfayli A, Darlenski R, et al. Glycerol and natural sugar-derived complex modulate differentially stratum corneum water-binding properties and structural parameters in an in vitro Raman-desorption model. J Biophotonics. 2023;16:e202200201. doi:10.1002/jbio.202200201.
4. Mäkeläinen H, Saarinen M, Stowell J, Rautonen N, Ouwehand AC. Xylo-oligosaccharides and lactitol promote the growth of Bifidobacterium lactis and Lactobacillus species in pure cultures. Beneficial Microbes. 2010;1(2):139-148. doi:10.3920/BM2009.0029.

PATENTS

1. WO1995015149A1 (1995) | Novel Cosmetic Compositions Containing Simple Polyols.
2. WO2006061678A1 (2006) | Cosmetic or Dermatological Compositions of Saccharose Substitutes.
3. US20240216265A1 (2024) | Liposomal Vegan Probiotic Skin Treatment.
4. WO2026167254A1 (2026) | Compositions Comprising Alditol and Alditol Derivatives for Supporting and Protecting the Skin.

NanoActive™ GalaBiome™ · PuriPharm Co. Ltd.

PuriActives® Ceramide E

The Advanced Pseudo-Ceramide Solution for Superior Skin Barrier Repair

PuriPharm Co. Ltd.

The Ceramide Challenge

Understanding the vital role of ceramides and the complex limitations of natural extraction in modern cosmetics.

The “Brick and Mortar” Model

The Epidermal Barrier
Ceramides account for over 50% of the stratum corneum lipids, acting as the essential ‘mortar’ holding the cellular ‘bricks’ together.

Moisture Retention
They uniquely form a structured lamellar network that powerfully binds water, actively preventing transepidermal water loss (TEWL).

Depletion Factors
Age, harsh chemicals, and environmental stressors rapidly deplete natural ceramides, inevitably leading to dryness, flaking, and irritation.

Natural Ceramide Limits

Natural ceramides are incredibly difficult and expensive to extract from plant or animal sources.

They exhibit notoriously poor solubility in both water and standard cosmetic oils.

Extremely high melting points (often over 90°C) make them challenging to formulate into stable emulsions.

Consequently, cosmetic formulators are often forced to use low, ineffective concentrations to prevent crystallization in the final product.

Introducing Ceramide E

A scientifically engineered, highly stable synthetic pseudo-ceramide developed by PuriPharm.

What is Ceramide E?

Scientific Profile
INCI: Cetyl-PG Hydroxyethyl Palmitamide.

It is a meticulously lab-created synthetic pseudo-ceramide designed to perfectly mimic the complex molecular structure and barrier function of natural epidermal sphingolipids.

The PuriActives® Advantage
It completely overcomes the formulation hurdles of natural ceramides. Ceramide E delivers identical barrier repair, profound hydration, and anti-inflammatory properties while remaining highly stable, safe, and cost-effective for modern skincare.

Replicating Nature

50% of Stratum Corneum Lipids

Biomimetic Structural Integrity

PuriActives® Ceramide E perfectly integrates into the skin’s natural lipid matrix. It acts as an identical substitute for the natural ceramides that naturally comprise half of your skin’s protective outer layer, ensuring long-lasting defense against environmental aggressors.

Mechanism of Action

Restoring the Lamellar Network

Ceramide E easily penetrates the upper layers of the epidermis, integrating directly into the compromised lipid matrix. It rapidly forms highly organized lamellar structures, effectively replacing lost intercellular “mortar”. This actively restores the structural integrity of the stratum corneum, locking in essential moisture while blocking external irritants.

Clinical Efficacies

Proven, measurable results in long-term skin hydration, structural protection, and barrier repair.

Core Skincare Benefits

Deep Hydration:
Massively enhances the skin’s capacity to retain moisture at a cellular level, dramatically improving overall elasticity, softness, and suppleness over continuous use.

Barrier Repair:
Actively replenishes the crucial lipid layer, effectively sealing microscopic epidermal cracks to stop transepidermal water loss (TEWL).

Soothing & Anti-Irritant:
Significantly reduces redness, flaking, and severe inflammation, making it highly recommended for sensitive, reactive, or eczema-prone skin types.

Anti-Aging Support:
Reinforces skin firmness and visibly minimizes the appearance of fine lines and wrinkles by maintaining optimal structural hydration and tissue resilience.

TEWL Reduction

Clinical applications demonstrate a dose-dependent, significant reduction in Transepidermal Water Loss (TEWL) compared to standard humectants, indicating a vastly fortified skin barrier.

24-Hour Hydration Trend

Unlike traditional humectants offering temporary relief, Ceramide E structurally locks in moisture, maintaining a significantly higher skin hydration index across a full 24-hour cycle.

Targeted Skin Conditions

Dry & Winter Skin
Eliminates flakiness and severe dryness by artificially rebuilding the compromised moisture barrier against harsh elements.

Sensitive & Atopic
Provides exceptional soothing relief for irritation, redness, and biologically compromised skin conditions like eczema.

Mature & Aging Skin
Combats the natural age-related decline of ceramide production, effectively restoring youthful firmness and tissue density.

Formulation Superiority

Formulators highly prefer PuriActives® Ceramide E as it completely bypasses the instability and crystallization risks of natural ceramides.

As a high-purity synthetic pseudo-ceramide, it offers exceptional batch consistency, prolonged shelf life, and effortless integration into complex cosmetic emulsions without degrading other actives.

Formulation & Applications

Technical guidelines and product versatility for cosmetic integration.

Versatile Applications

Facial Care
Essential for intensive barrier repair creams, high-end anti-aging serums, toners, and sensitive skin daily moisturizers.

Body Care
Highly effective in therapeutic body lotions, eczema-relief ointments, foot repair treatments, and winter care formulations.

Scalp & Hair
Excellent for anti-dandruff treatments, scalp soothing tonics, and deep conditioning hair masks to repair cuticle damage.

The Future of Barrier Care

“Synthetic pseudo-ceramides represent a critical breakthrough, delivering the profound barrier-repairing power of natural sphingolipids with unprecedented formulation stability.”

— Dermatological Research Perspectives

Formulation Process

1. Dispersion
Disperse the Ceramide E powder directly into the formulation’s designated oil phase.

2. Melting
Heat the entire oil phase to approximately 80°C to ensure complete dissolution.

3. Emulsion
Combine with the heated aqueous phase under high shear to form a stable emulsion.

4. Cooling
Cool the batch below 40°C before adding any heat-sensitive cosmetic actives.

Thank You

Discover the superior barrier repair of PuriActives® Ceramide E.

www.puriactives.com | sales@puripharm.com

UNLOCKING THE FULL POTENTIAL OF HEXYLRESORCINOL

HEXYLRESORCINOL: A MULTI-TARGET TONE-CORRECTION ACTIVE

POWERFUL BIOLOGY. DIFFICULT FORMULATION

WHY CONVENTIONAL HEXYLRESORCINOL CAN BE DIFFICULT TO USE

A DELIVERY-SYSTEM SOLUTION TO A MOLECULAR CHALLENGE

NANOACTIVE® HR: A FORMULATION-READY HEXYLRESORCINOL DELIVERY SYSTEM

BIOMIMETIC CARRIER. PROTECTED ACTIVE. OPTIMIZED INTERFACE

FROM FORMULATION LIABILITY TO DELIVERY ADVANTAGE

RECOMMENDED STABILITY VALIDATION PROGRAM

DESIGNED FOR MODERN SKINCARE FORMATS

HEXYLRESORCINOL ACTS BEYOND A SINGLE PIGMENTATION TARGET

PUBLISHED EVIDENCE SUPPORTS VISIBLE TONE IMPROVEMENT

STRONGER STRATEGIES THROUGH MULTI-PATHWAY DESIGN

A BROADER SKIN-QUALITY OPPORTUNITY

ONE ACTIVE. MULTIPLE PREMIUM PRODUCT STORIES

PROFESSIONAL INCORPORATION FOR CONSISTENT PERFORMANCE

DESIGNED FOR MULTI-PATHWAY FORMULATION STRATEGIES

NANOACTIVE® HR — TECHNICAL SPECIFICATIONS

FROM ACTIVE MOLECULE TO HIGH-PERFORMANCE DELIVERY SYSTEM

BRIGHTENING SCIENCE, DELIVERED MORE INTELLIGENTLY

SELECTED SCIENTIFIC AND TECHNICAL REFERENCES

Market Background

What Is Equol?

Why Equol for Skin Care?

Challenges of Conventional Equol

NanoActive® Delivery Platform

Product Concept

Core Benefits

Mechanism 1: Antioxidant Defense

Mechanism 2: ECM Support

Mechanism 3: Firmness & Elasticity

Mechanism 4: Photoaging Care

Mechanism 5: Soothing Care

Nano-Encapsulation Advantage

Formulation Applications

Formulation Guidelines

Claim Suggestions

Comparison with Other Anti-Aging Actives

Product Concept Formulas

PuriPharm Value Proposition

Safety & Regulatory Note

Scientific Background

References

A New Scientific Approach to Mature-Skin Care

The Mature-Skin Opportunity

What Is Equol?

Stereochemistry Matters

Why Estrogen-Related Pathways Matter in Skin Aging

A Receptor-Informed Approach to Skin Resilience

Breaking the Oxidative Stress–Matrix Degradation Cycle

Preserving the Architecture of Youthful-Looking Skin

Multi-Level Protection Against Oxidative Aging

Helping Skin Respond to the Modern Exposome

From Molecular Biomarkers to Skin-Relevant Pathways

Equol and Skin ECM Biomarkers

Clinical Evidence from Topical Equol Research

A Credible Evidence-to-Claim Framework

Turning a Potent Molecule into an Effective Formula

Formulating with PuriActives® EQUOL

Designed for Premium Skin-Longevity Concepts

From Ingredient Science to Consumer Innovation

A Distinctive Bioactive for Mature-Skin Care

Evidence-Based Claims for Global Development